Measurement

Part:BBa_K315043

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)


pLacI-RBS-RFP-RBS-TetA (Optimized from bp 1-290)

This sequence contains the wild type TetA gene which has been optimized from base pairs 1 to 290. This composite part has the construct of pLacI-RBS-RFP-RBS-TetA(Optimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is optimized, we are hypothesizing that it will allow for greater translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1129
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 1657
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLacI-RBS-RFP-RBS-TetA (Optimized from bp 1-290)